ANTIMONY SPECIATION
Although it has been used in medicine by man for several thousand years, antimony is found to be non-essential and toxic to human body. However, the toxicity of antimony is also expected to be specie and form dependent. Elemental antimony is more toxic than its salt, and trivalent Sb is generally ten times more toxic than pentavalent Sb species. Antimony trioxide is found to cause serious lung cancer by inhalation. Whereas some antimony containing salts are commonly used as therapeutic treatment of parasite such as leishmaniasis. The concentration and more importantly the speciation of antimony must be understood before its toxicity can be assessed.
PSA 10.825 ISOCRATIC LC SYSTEM FOR THE SPECIATION OF ANTIMONY
The PSA 10.055 Millennium Excalibur can easily be utilised for antimony
speciation by the simple addition of a liquid chromatography
system.
Separation of SbIII and SbV can be carried out in under 3 minutes using a simple isocratic elution program and a strong anion exchange column (PSA C1 or equivalent).
The system offers reliability and ease-of-use and gives limits of
detection of
0.2 ng ml-1,
making it a useful tool for any laboratory intested in antimony
speciation.
The system consists of a 1200 series Isocratic pump, manual injection valve and solvent cabinet. With the simple addition of the correct column, the analyser and hand held are ready to run. The 1200 series pump is made of robust materials to withstand the most demanding of applications; stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE. The seal wash option for high salt mobile phases keeps maintenance to a minimum, thereby providing lower operational costs.
The system can be enhanced by the C723I009 ChemStation base software. This software is the industries' most widely sold data system, handling all applications including LC, LC/MS, GC, CE and CE/MS. This base provides the ideal platform for labs that need an easy-to-learn and easy-to-use system, which can be scaled up and extended as the laboratory's needs grow.
Chromatogram of Five Consecutive Injection of 5 ng ml-1 Antimony Standards
The chromatogram below shows consecutive injections of 5 ng ml-1 mixed standards of Sb (V) and Sb (III). Species in real samples are identified on the basis of their retention times. Precision for the two species is below 5% RSD, and limits of detection for Sb (V) and Sb (III) are in the order of 0.7 and 0.5 ng ml-1, respectively. The chromatographic and chemical conditions are outlined in table 1. Two uncontaminated soil samples were extracted using mixed extraction solutions, stable species of Sb(V) and Sb(III) were obtained.
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For information on the speciation of other elements please follow the appropriate link below:
